affinity chromatography

Study on isolating complement C3d from the chicken serum by the method of immuno affinity chromatography

免疫亲和层析法分离鸡补体C3d蛋白研究

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Expressed proteins as inclusion bodies were solubilized , refolded and purified by GST affinity chromatography.

表达产物为包涵体形式,溶解和折叠后,以GST亲合层析色谱纯化.

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Affinity chromatography used to play important rule in the separation and purification of biomacromolecule.

亲和色谱多用于生物大分子的分离与纯化.

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Made sheep anti? human IgG Fab Sepharose 4 B affinity chromatography, purified the souble Fab fragment.

制备羊抗人 IgGFab 抗血清、建立抗人IgGFab抗体 -Sepharose4B亲和层析柱, 纯化Fab片段.

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The fusion protein was purified through Nickel-affinity chromatography column.

用镍柱纯化融合蛋白.

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Hepatitis delta antigen ( HDAg) from the liver of the woodchuck hepatitis virus ( WHV) carrier ( woodchuck infected with HDV) was purified through Sepharose 4B column and affinity chromatography column.

用丁型肝炎病毒（HDV）感染慢性携带土拨鼠肝炎病毒的东方土拨鼠（Woodchuck）后，取其肝组织制成匀浆，离心后经Sepharose4B柱，免疫亲和层析柱，获得高丰度的HDAg。

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Lectins in haemolymph of Sarcophaga peregrina larvae and pupa were purified by affinity chromatography.

用亲和层析法纯化了棕尾别麻蝇幼虫和蛹血淋巴凝集素.

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Affinity chromatography is one of the most important tools in protein research.

亲和层析技术是目前重要的蛋白质研究技术之一.

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Furthermore, the protein isolated in the periplasm can be purified by nickel affinity chromatography.

而且从周质空间分离的蛋白经过镍住得到了进一步的纯化.

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A lectin from the edible tomato ( Lycopersicon esculentum) was purified by hen ovomucoid-Sepharose 4B affinity chromatography.

番茄成熟果实经抽提上鸡卵类粘蛋白（OM）-Sepharose4B亲和层析柱分离纯化制得番茄凝集素。

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Objective To investigate the effect of position of hexahistine in recombinant protein on nickel affinity chromatography.

目的研究六聚组氨酸在重组蛋白中的位置对镍亲和层析的影响.

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The expressed product issoluble, MW 18 kD , which was purified by Ni + metal chelate affinity chromatography.

表达产物为可溶性蛋白,分子量约为18kD,通过Ni~+离子亲和层析一次性纯化.

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Recombinant human-like collagen withattacked to the carboxyl be easily purified by Metal Chelated Affinity Chromatography ( MCAC ) .

研究了应用金属螯合亲和 层析法 纯化重组类人胶原蛋白的条件.

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The supernant flows through Ni-NTA gel for affinity chromatography, and thepurity is over 91.5 %.

上清经Ni-NTA凝胶亲和层析, 纯度达到91.5%以上.

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A new methodology for preparation of ovine LH with high purity by anti-oLH_ β-IgG immuno-affinity chromatography has been reported. The biological activity of oLH is corresponding to 3,050 hCG IU/ mg, exhibiting a single band in PAGE.

本文报道用抗oLHβ-IgG免疫亲和层析柱纯化oLH的新方法，得到了生物学活性相当于3050·hCG IU/mg的oLH精制品，其电泳图谱显示高纯度的单一色带。

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Purpose To study purification method of the low molecular weight heparin ( LMWH ) by affinity chromatography.

目的探索亲和色谱纯化低分子 肝素 的方法.

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METHODS BALB/ c mice were immunized with human liver microsome proteins, and mAb was prepared by hybridoma technique. The crude mAb was purified by protein-G affinity chromatography.

方法：以含hCE-Ⅱ的人肝脏微粒体蛋白混合抗原免疫BALB/c小鼠，采用杂交瘤技术制备鼠抗hCE-Ⅱ的mAb，并用Protein-G亲和层析法纯化mAb。

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